Journal: International journal of molecular sciences
Article Title: Chronic Exposure to Chlorpyrifos Damages Thyroid Activity and Imbalances Hepatic Thyroid Hormones Signaling and Glucose Metabolism: Dependency of T3-FOXO1 Axis by Hyperglycemia.
doi: 10.3390/ijms24119582
Figure Lengend Snippet: Figure 8. Chlorpyriphos (CPF) effects on FOXO1-T3 axis in human hepatic carcinoma cells line and HepG2 cultured in normal- and high-glucose condition. (A–F) Representative Western blot of extracts from HepG2 cells cultured in both normal- and high-glucose condition (NG (A–C) and HG (D–F), respectively) and exposed to different doses of CPF (as detailed in Section 4), probed with p-FOXO1Aser256 and FOXO1A. Data were normalized for GAPDH protein. Western blots were quantified by densitometry using Image Lab Software. Results were obtained from three independent experiments and expressed as mean and ± SD. One-way ANOVA, ** p < 0.01, and *** p < 0.001 vs. CTRL. ELISA assay for fT3 in HepG2 extract cultured in NG (G) and HG (H) medium and treated with different CPF doses. Data represent the mean and the SD. Data represent three independent biological replicates. One-way ANOVA, ** p < 0.01.
Article Snippet: Human HepG2 hepatic carcinoma cell line was obtained from the American Type Culture Collection (ATCC).
Techniques: Cell Culture, Western Blot, Software, Enzyme-linked Immunosorbent Assay